Journal article
Data descriptor: Single cell RNA sequencing of stem cell-derived retinal ganglion cells
M Daniszewski, A Senabouth, QH Nguyen, DE Crombie, SW Lukowski, T Kulkarni, VM Sluch, JS Jabbari, X Chamling, DJ Zack, A Pébay, JE Powell, AW Hewitt
Scientific Data | NATURE PUBLISHING GROUP | Published : 2018
Abstract
We used single cell sequencing technology to characterize the transcriptomes of 1,174 human embryonic stem cell-derived retinal ganglion cells (RGCs) at the single cell level. The human embryonic stem cell line BRN3B-mCherry (A81-H7), was differentiated to RGCs using a guided differentiation approach. Cells were harvested at day 36 and prepared for single cell RNA sequencing. Our data indicates the presence of three distinct subpopulations of cells, with various degrees of maturity. One cluster of 288 cells showed increased expression of genes involved in axon guidance together with semaphorin interactions, cell-extracellular matrix interactions and ECM proteoglycans, suggestive of a more ma..
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Grants
Awarded by National Institute of General Medical Sciences
Funding Acknowledgements
This study was supported by the National Program for Support of Top-Notch Young Professionals, the STU Scientific Research Foundation for Talents (No. NTF17006), the National Natural Science Foundation of China (No. 31772837), and the "Sail Plan" Program for the Introduction of Outstanding Talents of Guangdong Province, China.